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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -AFX, phosphorylated (Ser193) (Forkhead Box Protein O4, Fork Head Domain Transcription Factor AFX1, FOXO4, AFX1, MLLT7)

Anti -AFX, phosphorylated (Ser193) (Forkhead Box Protein O4, Fork Head Domain
Transcription Factor AFX1, FOXO4, AFX1, MLLT7)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family, three members (FKHR, AFX, AND FKHRL1) have sequence similarity to the nematode homolog Daf 16, recently shown to mediate signaling via a pathway involving IGFR1, P13-K and Akt. There are three potential Akt phosphorylation sites on the FKHR proteins. One site, FKHR Ser256 is found in the Forkhead domain and the other two are outside this domain. Recent studies show that phosphorylation of FKHR family members by Akt promotes cell survival. Phosphorylation regulates FKHR nuclear translocation and target gene transcription. Insulin also stimulates FKHR phosphorylation at Ser253.
Catalog #A0928-02F
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 1% BSA, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to residues surrounding Ser193 from human AFX (AAI06762.1).
SpecificityRecognizes human AFX when phosphorylated at Ser193. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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