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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH

Several studies indicate that FKHR and AFX, mammalian homologues of the Caenorhabditis elegans forkhead transcription factor DAF-16, function in the insulin signaling pathway promoter contains one IRS, while in the IGFBP-1 promoter two copies of IRS are arranged as an inverted palindrome. Three tandem copies of IRSs are present in the G6Pase. AFX has been recently shown to serve as a substrate of PKB both in vitro and in vivo, and its transcriptional activity is inhibited by phosphorylation.
Catalog #	A0928-03
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:500-1:1000
	Immunohistochemistry: 1:50-1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthesized non-phosphopeptide derived from human AFX around the phosphorylation site of serine 197
Specificity	Detects endogenous levels of total AFX protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.