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You are here:Home » Lectins » Lectin-Agaricus bisporus » Agaricus bisporus (Mushroom) (ABA) (Biotin)

Agaricus bisporus (Mushroom) (ABA) (Biotin)

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Specifications

Lectins are derived from the extracts of plants, animals, viruses and microorganisms. They are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.
Catalog #A0980-10
Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.
Specificity Carbohydrate Specificity: Gal(b1,3)GalNAc
Inhibitory Carbohydrate b-D-galactose (normally). Recognizes the structure Gal-GalNAc-serine (or threonine).
ApplicationsSuitable for use in Agglutination. Other applications not tested.
Recommended DilutionsAgglutination of type A or B cells: 5-10ug
Agglutination of type O cells: 2ug/ml
Agglutination of neuraminidase treated erythrocytes: < 0.5ug/ml
Optimal dilutions to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight58.5kD
SourceMushroom
PurityHighly purified (SDS-PAGE)
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.4. Labeled with Biotinyl N-hydroxysuccinimide ester (BNOHSA, Biotin).
ReconstitutionReconstitute with 1ml sterile dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesmushroom


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