| Agarose for DNA Fragments 50-1000bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels. |
|
| Catalog # | A1005 |
| Manufactured specifically for primer size fragments in the range of 50-1000 base pairs; exhibits exceptionally high transparency even at high concentrations (4.5%). |
| Applications | • Analytical DNA Gels 1000bp |
| • PCR products |
| • Restriction Enzyme digestion products |
| • Mutation Analysis |
| • Blotting Assays 600bp |
| • For use with all buffer systems |
| Recommended Usage (1X TAE) | Concentration Molecular Weight |
| 1.8% 500-1000 bp |
| 3.0% 150-600 bp |
| 4.5% 50-350 bp |
| Note: A 3% agarose gel is equivalent to 8% polyacrylamide |
| Storage and Stability | Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use. |
| CAS Number | [9012-36-6] |
| Appearance | White, fine, homogenous, free flowing powder |
|
| EEO | 0.13 |
| Clarity (1.5%, NTU) | ~4 |
| Absorbance (430nm, 1.5%) | 0.040 |
| pH (1.5%, H2O, Solution) | 6-7 |
| pH (1.5%, H2O, Gel) | 5.0-7.0 |
|
| Loss on Drying | 7.0% |
| Ash | 0.35% |
| Sulfate | 0.1% |
|
| Melting Point (3%) | 75°C |
| Gel Point (3%) | 35°C |
| Gel Strength (1.5%) | 750g/cm2 |
| Gel Strength (3%) | 1500g/cm2 |
|
| RNase | None Detected |
| DNase | None Detected |
|
| Quality Control | Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. |
|
| Functional and Molecular Biology Testing | To Pass Test |
| • Comparative assay of different size DNA fragments |
| • Southern Blot (125-23,000bp) |
| • Background Fluorescence (TAE/TE, EtBr) | |
| • DNA Binding (TAE, EtBr) |
| • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
