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You are here:Home » BioSeparation? » BioSep-Agarose » Agarose DNA Fragments 50-1000bp

Agarose DNA Fragments 50-1000bp

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Specifications

Agarose for DNA Fragments 50-1000bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels.
Catalog #A1005
Manufactured specifically for primer size fragments in the range of 50-1000 base pairs; exhibits exceptionally high transparency even at high concentrations (4.5%).
Applications Analytical DNA Gels 1000bp
PCR products
Restriction Enzyme digestion products
Mutation Analysis
Blotting Assays 600bp
For use with all buffer systems
Recommended Usage (1X TAE)Concentration Molecular Weight
1.8% 500-1000 bp
3.0% 150-600 bp
4.5% 50-350 bp
Note: A 3% agarose gel is equivalent to 8% polyacrylamide
Storage and Stability Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.
CAS Number9012-36-6
AppearanceWhite, fine, homogenous, free flowing powder
EEO0.13
Clarity (1.5%, NTU)~4
Absorbance (430nm, 1.5%)0.040
pH (1.5%, H2O, Solution)6-7
pH (1.5%, H2O, Gel)5.0-7.0
Loss on Drying7.0%
Ash0.35%
Sulfate0.1%
Melting Point (3%)75°C
Gel Point (3%)35°C
Gel Strength (1.5%)750g/cm2
Gel Strength (3%)1500g/cm2
RNaseNone Detected
DNaseNone Detected
Quality ControlGenetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
Functional and Molecular Biology TestingTo Pass Test
Comparative assay of different size DNA fragments
Southern Blot (125-23,000bp)
Background Fluorescence (TAE/TE, EtBr)
DNA Binding (TAE, EtBr)
Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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