| Agarose for DNA Fragments 150-1500bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels. |
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| Manufactured specifically for primer size fragments in the range of 150-1500 base pairs and exhibits exceptionally high transparency even at high concentrations (4.5%). |
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| Catalog # | A1010 |
| Applications | • Analytical DNA Gels 1500bp |
| • PCR products |
| • Restriction Enzyme digestion products |
| • Mutation Analysis |
| • Blotting Assays 600bp |
| • For use with all buffer systems |
| Recommended Usage (1X TAE) | Concentration Molecular Weight |
| 4% 150-600 bp |
| 12% 500-1500 bp |
| Storage and Stability | Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use. |
| CAS Number | 9012-36-6 |
| Appearance | White, fine, free flowing powder |
| Clarity (NTU, 1.5%) | ~1.5 |
| Colorimetry (A450) | 0.02 |
| pH in solution (1.5%) | 5.5-7.0 |
| pH in gel (1.5%) | 5.5-7.0 |
| Gel Strength (1.5%) | 2000g/cm2 |
| Gel Strength (4%) | 4250g/cm2 |
| Gel Point (4%) | 40°C |
| Melting Point (4%) | 89ºC |
| EEO | 0.12 |
| Loss on Drying | 7.0% |
| Ash | 0.35% |
| Sulfate | 0.1% |
| RNase | None Detected |
| DNase | None Detected |
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| Quality Control | Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. |
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| Functional and Molecular Biology Testing | To Pass Test |
| • Comparative assay of different size DNA fragments |
| • Southern Blot (125-23,000bp) |
| • Background Fluorescence (TAE/TE, EtBr) | |
| • DNA Binding (TAE, EtBr) |
| • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
