| USBio's Agarose for DNA Fragments 50-2000bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels. |
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| Catalog # | A1012 |
| Manufactured specifically for primer size fragments in the range of 50-2000 base pairs and exhibits exceptionally high transparency even at high concentrations (5%). |
| Specially purified to be free of background contamination that could interfere with restriction digests or ligations and selected for use in PCR product analysis. |
| Applications | • Analytical DNA Gels 2000bp |
| • PCR products |
| • Restriction Enzyme digestion products |
| • Capillary Electrophoresis |
| • Mutation Analysis |
| • For use with all buffer systems |
| Recommended Usage (1X TAE) | Concentration Molecular Weight |
| 1-4% 50-2000bp |
| 1% 500-2000bp |
| 2% 250-750bp |
| 3% 125-500bp |
| 4% 50-250bp |
| Note: A 3% agarose gel is equivalent to 8% polyacrylamide |
| Storage and Stability | Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use. |
| CAS Number | [9012-36-6] |
| EEO | 0.12 |
| Melting Point (1.5%) | 89°C |
| Gel Point (1.5%) | 36.5°C |
| Gel Strength (4%) | 4250g/cm2 |
| Absorbance (655nm,1.5%) | 0.008 |
| Sulfate | 0.1% |
| RNase | None Detected |
| DNase | None Detected |
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| Quality Control | Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. |
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| Functional and Molecular Biology Testing | To Pass Test |
| • Comparative assay of different size DNA fragments |
| • Southern Blot (125-23,000bp) |
| • Background Fluorescence (TAE/TE, EtBr) | |
| • DNA Binding (TAE, EtBr) |
| • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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