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You are here:Home » Molecular Biology » MB-BioSep Agarose » Agarose DNA Fragments 50-2000bp (3:1 Blend)

Agarose DNA Fragments 50-2000bp (3:1 Blend)

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Specifications

Agarose for DNA Fragments 50-2000bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels.
Catalog #A1012
Manufactured specifically for primer size fragments in the range of 50-2000 base pairs and exhibits exceptionally high transparency even at high concentrations (5%).
Specially purified to be free of background contamination that could interfere with restriction digests or ligations and selected for use in PCR product analysis.
Applications Analytical DNA Gels 2000bp
PCR products
Restriction Enzyme digestion products
Capillary Electrophoresis
Mutation Analysis
For use with all buffer systems
Recommended Usage (1X TAE)Concentration Molecular Weight
1-4% 50-2000bp
1% 500-2000bp
2% 250-750bp
3% 125-500bp
4% 50-250bp
Note: A 3% agarose gel is equivalent to 8% polyacrylamide
Storage and Stability Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.
CAS Number9012-36-6
Appearance
White. granular, free flowing powder
EEO
0.12
Absorbance (450nm,1.5%)
As reported
Melting Temperature
93°C
Gelling Temperature
40.5°C
Gel Strength (1.5%)
2000g/cm2
Gel Strength (4%)
4250g/cm2
pH in Solution (1.5%,H2O)
5.0-7.0
Loss on Drying
7.0%
Sulfate
0.1%
Ash
0.35%
RNase
None Detected
DNase
None Detected
DNA Binding
To Pass Test
Background Fluorescence (EtBr)
To Pass Test
Quality ControlGenetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
Functional and Molecular Biology TestingTo Pass Test
Comparative assay of different size DNA fragments
Southern Blot (125-23,000bp)
Background Fluorescence (TAE/TE, EtBr)
DNA Binding (TAE, EtBr)
Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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