| USBio's Agarose for DNA Fragments 50-2000bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels. |
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| Catalog # | A1012 |
| Manufactured specifically for primer size fragments in the range of 50-2000 base pairs and exhibits exceptionally high transparency even at high concentrations (5%). |
| Specially purified to be free of background contamination that could interfere with restriction digests or ligations and selected for use in PCR product analysis. |
| Applications | • Analytical DNA Gels 2000bp |
| • PCR products |
| • Restriction Enzyme digestion products |
| • Capillary Electrophoresis |
| • Mutation Analysis |
| • For use with all buffer systems |
| Recommended Usage (1X TAE) | Concentration Molecular Weight |
| 1-4% 50-2000bp |
| 1% 500-2000bp |
| 2% 250-750bp |
| 3% 125-500bp |
| 4% 50-250bp |
| Note: A 3% agarose gel is equivalent to 8% polyacrylamide |
| Storage and Stability | Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use. |
| CAS Number | 9012-36-6 |
| Appearance | |
| White. granular, free flowing powder |
| EEO | |
| 0.12 |
| Absorbance (450nm,1.5%) | |
| As reported |
| Melting Temperature | |
| 93°C |
| Gelling Temperature | |
| 40.5°C |
| Gel Strength (1.5%) | |
| 2000g/cm2 |
| Gel Strength (4%) | |
| 4250g/cm2 |
| pH in Solution (1.5%,H2O) | |
| 5.0-7.0 |
| Loss on Drying | |
| 7.0% |
| Sulfate | |
| 0.1% |
| Ash | |
| 0.35% |
| RNase | |
| None Detected |
| DNase | |
| None Detected |
| DNA Binding | |
| To Pass Test |
| Background Fluorescence (EtBr) | |
| To Pass Test |
|
| Quality Control | Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. |
|
| Functional and Molecular Biology Testing | To Pass Test |
| • Comparative assay of different size DNA fragments |
| • Southern Blot (125-23,000bp) |
| • Background Fluorescence (TAE/TE, EtBr) | |
| • DNA Binding (TAE, EtBr) |
| • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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