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You are here:Home » BioSeparation? » BioSep-Agarose » Agarose Low EEO (MB)

Agarose Low EEO (MB)

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Specifications

Agarose Low EEO Molecular Biology Grade is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels. Low EEO Agarose is recommended for a wide array of molecular biology procedures and offers enhanced resolution ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
Catalog #A1015
Applications Analytical DNA Gels 1000bp
Blotting Assays (Southern, northern)
Radial Immunodiffusion
Restriction Enzyme digestion products
For use with all buffer systems
Recommended Usage (0.5X TBE)Concentration Molecular Weight
1% 500bp-10kb
1.2% 350bp-7kb
1.5% 250bp-2kb
Storage and Stability Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.
CAS Number9012-36-6
AppearanceWhite, homogeneous, free flowing powder
EEO (pH 8.4, Wieme Method)0.09-0.13
Loss on Drying7.0%
Melting Point(1.5%)86.5-89.5°C
Gel Point (1.5%)34.5-37.5°C
Gel Strength (1.5%)2500g/cm2
Sulfate0.20%
Ash0.50%
Clarity (1.5%)40 NTU
pH, Solution7.0 ± 0.5
pH, Gel6.5 ± 0.5
Absorbance (450nm)As reported
DNase and RNaseNone Detected
Quality ControlGenetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
Functional and Molecular Biology TestingTo Pass Test
Comparative assay of different size DNA fragments
Southern Blot (125-23,000bp)
Background Fluorescence (TAE/TE, EtBr)
DNA Binding (TAE, EtBr)
Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesFargo


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