| Agarose Low EEO Electrophoresis Grade is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels. Low EEO Agarose is recommended for a wide array of molecular biology procedures and offers enhanced resolution ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. |
|
| Catalog # | A1016 |
| See A1015 for our best grade with complete Functional and Molecular Biology Testing. |
| Applications | • Analytical DNA Gels 1000bp |
| • Blotting Assays (Southern, northern) |
| • Radial Immunodiffusion |
| • Restriction Enzyme digestion products |
| • For use with all buffer systems |
| Recommended Usage (0.5X TBE) | Concentration Molecular Weight |
| 1% 500bp-10kb |
| 1.2% 350bp-7kb |
| 1.5% 250bp-2kb |
| Storage and Stability | Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use. |
| CAS Number | [9012-36-6] |
| Appearance | White, fine, homogeneous, free flowing powder |
| EEO (pH 8.4, Wieme Method) | 0.09-0.13 |
| Loss on Drying | 7.0% |
| Melting Point(1.5%) | 86.5-89.5°C |
| Gel Point (1.5%) | 34.5-37.5°C |
| Gel Strength (1.5%) | 2500g/cm2 |
| Sulfate | 0.20% |
| Ash | 0.50% |
| Clarity (1.5%) | 40 NTU |
| pH, Solution | 7.0 ± 0.5 |
| pH, Gel | 6.5 ± 0.5 |
| Absorbance (450nm) | As reported |
|
| Quality Control | Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. |
|
| Functional and Molecular Biology Testing | To Pass Test |
| • Comparative assay of different size DNA fragments |
| • Southern Blot (125-23,000bp) |
| • Background Fluorescence (TAE/TE, EtBr) | |
| • DNA Binding (TAE, EtBr) |
| • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
