| Suitable for protein and large DNA separations 1000bp. The low melting temperature allows for the recovery, without damage, of nucleic acids below their denaturing temperatures. |
|
| Catalog # | A1020 |
| The low melting point also ensures that the agarose will be in a liquid state where in-gel manipulations can be performed without prior purification of the DNA from the gel slice. |
| Applications | • Analytical DNA Gels 1000bp |
| • Blotting Assays (Southern, northern) |
| • Restriction Enzyme Digestion |
| • Ligation |
| • DNA Labeling |
| • Nick Translation |
| • Random Priming |
| • PCR |
| • Tissue Culture |
| • Viral Plaque |
| • For use with all buffer systems |
| Recommended Usage (0.5X TBE) | Concentration Molecular Weight |
| 1% 250-10,000bp |
| 1.5% 150-2500bp |
| Storage and Stability | Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use. |
| CAS Number | [9012-36-6] |
| EEO | 0.12 |
| Melting Point (1.5%) | 65.5°C |
| Gel Point (1.5%) | 24-28°C |
| Gel Strength (1.5%) | 500g/cm2 |
| Sulfate | 0.1% |
| RNase | None Detected |
| DNase | None Detected |
|
| Quality Control | Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. |
|
| Functional and Molecular Biology Testing | To Pass Test |
| • Comparative assay of different size DNA fragments |
| • Southern Blot (125-23,000bp) |
| • Background Fluorescence (TAE/TE, EtBr) | |
| • DNA Binding (TAE, EtBr) |
| • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
