| High Gel Strength Agarose Combines very high gel strength with low EEO and is a good choice for high speed, high temperature pulsed field gel electrophoresis applications. |
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| Catalog # | A1025 |
| Applications | • Pulsed Field Electrophoresis |
| • Immunoelectrophoresis |
| • Blotting Assays (Southern, northern) |
| • Analytical DNA Gels 1000bp |
| • Preparative Electrophoresis |
| • For use with all buffer systems |
| Recommended Usage (1X TAE) | Concentration Molecular Weight |
| 0.4-2.0% 50-500kb |
| Storage and Stability | Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use. |
| CAS Number | 9012-36-6 |
| EEO | 0.14 |
| Melting Point (1.5%) | 85.5-88.5°C |
| Gel Point (1.5%) | 39.5-42.5°C |
| Gel Strength (1.5%) | 1200g/cm2 |
| Clarity | 50 NTU |
| Sulfate | 0.2% |
| RNase | None Detected |
| DNase | None Detected |
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| Quality Control | Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. |
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| Functional and Molecular Biology Testing | To Pass Test |
| • Comparative assay of different size DNA fragments |
| • Southern Blot (125-23,000bp) |
| • Background Fluorescence (TAE/TE, EtBr) | |
| • DNA Binding (TAE, EtBr) |
| • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
