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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Aggrecan G1-IGD-G2 Domains (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, M

Anti -Aggrecan G1-IGD-G2 Domains (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified
by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate
proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, M

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
As the key component of the cartilage extracellular matrix, aggrecan hydrates the collagen network and provides cartilage with its properties of compressibility and elasticity. Maintenance of aggrecan content is therefore critical to the function of the tissue and aggrecan degradation is an important factor in the erosion of articular cartilage in arthritic diseases. The deduced amino acid sequence of human aggrecan core protein consists of 2415 resides and predicts a signal peptide and domains of G1, IGD, G2, KS, CS-1, CS-2, and G3. Two globular domains, G1 and G2, comprise the N­terminus of the proteoglycan and also contain link domains. The third globular domain, G3, corresponds to the C-terminus. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains are between G2 and G3. With KS and CS attached to the 250kD core protein, aggrecan monomers exist as a 1000-2000 kD molecule. In addition, aggrecan monomers interact with hyaluronan through their G1 domain, resulting in larger aggregates containing 10-100 aggrecan monomers on a hyaluronan backbone.
Catalog #A1059-53H
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry: 15ug/ml detects aggrecan in calls and tissues.
Western Blot: 0.1-0.2ug/ml the detection limit is ~5ng/lane under reducing and non-reducing conditions.
Direct ELISA: 0.5-1ug/ml the dection limit is ~0.5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa20-675 from human Aggrecan G1-IGD-G2 Domains, expressed in NS0 cells (NP_037359).
SpecificityRecognizes human Aggrecan G1-IGD-G2 Domains.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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