Anti -Aggrecan (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal
A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core
protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Supernatant |
E B IH |
|
| This aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue. | | | Catalog # | A1059-53S | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:50 | | Immunohistochemistry: formalin or paraformalin paraffin embedded sections or alcohol-fixed frozen or unfixed snap-frozen sections. | | Notes: | | Samples must be deglycosylated using 0.01 Units Chondroitinase ABC, 0.01 Units Keratanase and 0.0001 Units Keratanase II per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. see Little et al. and Caterson et al. Diluted Technomouse culture supernatant. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 8J207 | | Host | Mouse | | Concentration | Not determined | | Form | Supplied as a liquid in 0.2M Tris/HCl pH 7.4, 1-10% fetal calf serum, 0.02% sodium azide. | | Purity | Supernatant | | Immunogen | C-terminal neoepitope, TEGE after cleavage between amino acids, EGE373 and 374ARG, within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration) | | Specificity | Recognizes the aggrecanase (ADAMTS-1, -4 & -5). Species Crossreactivity: human, porcine, bovine, equine and canine tissues. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Aggrecan (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK) |
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