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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Aggrecan ARGxx (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK)

Anti -Aggrecan ARGxx (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by
monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate
proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16,
SEDK)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E B IH
Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.
Catalog #A1059-53U
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:50 Detects a variety of epitopes between 50 and 250kD.
Immunohistochemistry: Formalin or paraformaldehyde- fixed paraffin embedded sections or alcohol-fixed frozen or unfixed snap-frozen sections.
Notes:Samples must be deglycosylated using 0.01 Units Chondroitinase ABC, 0.01 Units Keratanase and 0.0001 Units Keratanase II per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. Diluted Technomouse culture supernatant.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No8J209
HostMouse
ConcentrationNot Determined
FormSupplied as a liquid in 0.2M Tris/HCl pH 7.4, 01-10% fetal calf serum, 40% glycerol.
PurityTissue culture supernatant
ImmunogenN-terminal neoepitope ARG after cleavage between aa EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration).
SpecificityRecognizes the aggrecanase (ADAMTS-1, -4, -5). Detects a variety of epitopes between 50 and 250kD. Species Crossreactivity: human, bovine, feline, canine, guinea pig, equine, porcine, rabbit, rat and sheep. Does not react with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesAGC 1, AGC1, AGC1 protein, Aggrecan 1, Aggrecan core protein, Aggrecan1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG


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