Anti -Aggrecan ARGxx (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by
monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate
proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16,
SEDK)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Supernatant |
E B IH |
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| Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease. | | | Catalog # | A1059-53U | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:50 Detects a variety of epitopes between 50 and 250kD. | | Immunohistochemistry: Formalin or paraformaldehyde- fixed paraffin embedded sections or alcohol-fixed frozen or unfixed snap-frozen sections. | | Notes:Samples must be deglycosylated using 0.01 Units Chondroitinase ABC, 0.01 Units Keratanase and 0.0001 Units Keratanase II per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. Diluted Technomouse culture supernatant. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 8J209 | | Host | Mouse | | Concentration | Not Determined | | Form | Supplied as a liquid in 0.2M Tris/HCl pH 7.4, 01-10% fetal calf serum, 40% glycerol. | | Purity | Tissue culture supernatant | | Immunogen | N-terminal neoepitope ARG after cleavage between aa EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration). | | Specificity | Recognizes the aggrecanase (ADAMTS-1, -4, -5). Detects a variety of epitopes between 50 and 250kD. Species Crossreactivity: human, bovine, feline, canine, guinea pig, equine, porcine, rabbit, rat and sheep. Does not react with mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | AGC 1, AGC1, AGC1 protein, Aggrecan 1, Aggrecan core protein, Aggrecan1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG |
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Download Specifications for Anti -Aggrecan ARGxx (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK) |
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