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You are here:Home » Antibodies » Abs to Biochemicals » Anti -Agmatine (Guanidinium Analogue)

Anti -Agmatine (Guanidinium Analogue)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH IC
Catalog #A1059-56
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (paraffin): 1:100 for 0.1-2.5% glutaraldehyde fixed tissues. Optimal fixation: 0.1-2.5% glutaraldehyde, 1% formalin. Minimum glutaraldehyde: 0.1%; works on paraffin embedded tissue (fixed with glutaraldehyde) but preferably epoxy embedded specifically developed for post-embedding protocols. Living, isolated goldfish retinas were maintained for 10 minutes in an oxygenated physiological solution that preserves normal neuronal activity under constant lighting. The medium also contained 10mM agmatine. After the incubation period retinas were fixed in a conventional 2.5% glutaraldehyde buffer and processed for immunohistochemistry using 250nm sections probed with a gold-labeled secondary antibody. The treated retinas were exposed to 125M kainate during the incubation. Kainate opens both AMPA and kainate selective ionotropic channels through which agmatine can enter cells.
Immunocytochemistry: On cells with glutamate-gated ion channels.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 1% goat serum, 0.05% Thermasol.
PuritySerum
ImmunogenAgmatine (BSA coupled)
SpecificityRecognizes Agmatine. Does not crossreact with arginine, glutamate or other amino acids. Is expected to recognize agmatine in mose vertebrate species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names(4-aminobutyl)guanidine


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