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You are here:Home » Molecular Biology » MB-Proteins » Agouti (Control Peptide)

Agouti (Control Peptide)

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Specifications

Control peptide for A1059-58 (anffinity purified antibody) and A1059-59B (antiserum).
Catalog #A1059-58A
Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132aa in human) is normally expressed in skin. Its ubiquitous expression, such as in rare allele lethal yellow, causes yellow coat., linear growth, immune defects and obesity. Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1 and possibly MC2R and MC4R).
ApplicationsSuitable for use in Antibody Blocking, ELISA. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended DilutionAntibody Blockingt: 5-10ug per 1ug A1059-58 (anffinity purified antibody) or 1ul A1059-59B (antiserum).
ELISA: 50-100ng control peptide per well.
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityPurified
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
SpecificitySynthetic peptied consisting of 21aa near the N-terminus of mouse Agouti. No sequence homology is seen with AGRP, tubby, tub, TULP1 and TULP2. Species Sequence Homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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