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You are here:Home » Antibodies » Abs to Proteins » Anti -Agouti Protein

Anti -Agouti Protein

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132aa in human) is normally expressed in skin. Its ubiquitous expression, such as in rare allele lethal yellow, causes yellow coat., linear growth, immune defects and obesity. Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1 and possibly MC2R and MC4R).
Catalog #A1059-59B
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 (ECL).
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide (A1059-58A) per well.
Optimal dilutions to be determined by researcher.
Control PeptideA1059-58A: Agouti (Control Peptide)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide consisting of 21aa near the N-terminus of mouse Agouti, conjugated to KLH. Species Sequence Homology: rat 90%, human and monkey 80%, fox 75%, porcine 73% and Bovine 66%.
SpecificityRecognizes mouse Agouti. No homology is seen with AGRP, tubby, tub, TULP1 and TULP2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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