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You are here:Home » Antibodies » Antibodies-Obesity Proteins » Anti -AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript)

Anti -AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Catalog #A1059-62B
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA (Direct): 0.5-1.0ug/ml well. The detection limit for recombiant human AgRP is ~6ng/ml.
Western Blot: 1-2ug/ml. The detection limit for recombinant human AgRP is ~25ng/lane under non-reducing and reducing conditions using colorimetric detection system.
Optimal dilutions to be determined by the researcher.
HybridomaMyeloma with B cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9H47
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to human Agouti-Related Protein, expressed in Sf 21 cells (NP_015531).
SpecificityRecognizes human AgRP. Shows ~10% crossreactivity with recombinant mouse AgRP in direct ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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