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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript)

Anti -AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1). However, recombinant Agouti protein also antagonizes the MC2R and MC4R. AGRP (132 aa in human, chromosome 16q21) is normally expressed in adrenal and hypothalamus. AGRP levels are increased several folds in ob/ob mice. AGRP is a strong antagonist of MC3R and MC4R. Ubiquitous expression of AGRP in transgenic mice causes obesity without altering skin pigmentation.
Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132 aa in human) is normally expressed in skin but its ubiquitous expression causes obesity.
Catalog #A1059-62D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000.
ELISA: 1:10,000-100,000.
Optimal dilutions to be determined by the researcher.
Control Peptide A1059-62A AGRP, Mouse Control Peptide
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid, PBS, 0.05% sodium azide, 40% glycerol.
PuritySerum
ImmunogenSynthetic peptide corresponding to 15aa sequence near C-terminus of mouse AGRP (KLH).
SpecificityRecognizes mouse Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript. Species sequence homology: human and bovine (85%). No homology is seen with Agouti, tubby, tub, TULP1 and TULP2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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