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Anti -AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript) (Biotin)
Anti -AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| Produced in goats immunized with purified, insect cell line Sf 21-derived, recombinant human Agouti-Related Protein (rhAgRP), also known as the protein product of Agouti-related transcript (ART). AgRP specific IgG was purified by human AgRP affinity chromatography and then biotinylated. | | | Catalog # | A1059-62M | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: Can be used as a detection antibody in a human AgRP ELISA in combination with the monoclonal capture antibody (Catalog# A1059-62G). Using plates coated with 100ul/well of the capture antibody, in combination with 100ul/well of the detection antibody at 50ng/ml, an ELISA for sample volumes of 100ul can be obtained. To arrive at the optimal dose range for | | this ELISA, set up a two-fold dilution series of the protein standard stAgRPing with 4ng/ml. | | Western Blot: 0.1-0.2ug/ml. The detection limit for rhAgRP is approximately 1ng/lane under non-reducing and reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~50ug/ml | | Form | Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 50ug BSA. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base, 150mM sodium chloride) containing 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml. | | Purity | Purified by immunoaffinity chromatography. | | | Immunogen | Sf 21-derived, recombinant human Agouti-Related Protein. | | Specificity | Recognizes human AgRP. ~40% cross-reactivity with rmAgRP. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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