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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Agouti-Related Protein (AGRP)

Anti -Agouti-Related Protein (AGRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
The Agouti-related protein (Agrp) -- secreted hypothalamic factors regulated by the metabolic state in mammals -- is involved in energy homeostasis and plays an important role in the control of feeding behavior. Agouti protein and Agouti-related protein are paracrine-signaling molecules that normally regulate pigmentation and body weight. The mature human protein is a single polypeptide chain of 112 amino acid residues consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. Circular dichroism studies indicated that the purified protein contains primarily random coil and beta-sheet secondary structure. The hypothalamic expression of Agrp is regulated by leptin, and overexpression of Agrp in transgenic animals results in obesity and diabetes.
Catalog #A1059-63A
ApplicationsSuitable for use in Indirect ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Amino Acid Sequence; MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 50ul sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant protein corresponding to 128aa with a N-teminal His-tag from human Agouti-related protein expressed in E. coli.
SpecificityRecognizes human Agouti-Related Protein (AGRP) at ~14.4kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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