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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

The Agouti-related protein (Agrp) -- secreted hypothalamic factors regulated by the metabolic state in mammals -- is involved in energy homeostasis and plays an important role in the control of feeding behavior. Agouti protein and Agouti-related protein are paracrine-signaling molecules that normally regulate pigmentation and body weight. The mature human protein is a single polypeptide chain of 112 amino acid residues consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. Circular dichroism studies indicated that the purified protein contains primarily random coil and beta-sheet secondary structure. The hypothalamic expression of Agrp is regulated by leptin, and overexpression of Agrp in transgenic animals results in obesity and diabetes.
Catalog #	A1059-63A
Applications	Suitable for use in Indirect ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
	Amino Acid Sequence;MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 50ul sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Recombinant protein corresponding to 128aa with a N-teminal His-tag from human Agouti-related protein expressed in E. coli.
Specificity	Recognizes human Agouti-Related Protein (AGRP) at ~14.4kD.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.