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You are here:Home » Antibodies » Abs to Apoptosis » Anti -AIF (Apoptosis Inducing Factor, Harlequin, Hq, mAIF, MGC111425, MGC5706, Programmed Cell Death 8, Programmed Cell Death Protein 8 Mitochondrial Precursor, PDCD8, Striatal Apoptosis Inducing Factor)

Anti -AIF (Apoptosis Inducing Factor, Harlequin, Hq, mAIF, MGC111425, MGC5706, Programmed
Cell Death 8, Programmed Cell Death Protein 8 Mitochondrial Precursor, PDCD8, Striatal
Apoptosis Inducing Factor)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B IH
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and widely expressed (2).
Catalog #A1059-75U
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.25-1ug/ml. A band at ~67kD
Immunohistochemistry:10ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlK562 cell lysates
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified
ImmunogenSynthetic peptide corresponding to aa517-531 of human AIF. This sequence is identical to those of mouse and rat AIF.
SpecificityRecognizes human AIF. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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