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You are here:Home » Antibodies » Abs to Apoptosis » Anti -Apoptosis Inducing Factor (AIF, MGC111425, Programmed cell death protein 8, mitochondrial precursor, PDCD8)

Anti -Apoptosis Inducing Factor (AIF, MGC111425, Programmed cell death protein 8,
mitochondrial precursor, PDCD8)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #A1059-77
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot Analysis: 1ug/ml detects AIF from a mouse liver preparation. Mouse liver protein preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with AIF (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Western Blot Protocol 1. Perform SDS-PAGE on a sample and transfer the proteins to nitrocellulose. Wash the blotted nitrocellulose twice with water. 2. Block the blotted nitrocellulose in freshly prepared TBS containing 3% nonfat dry milk with 0.1% Tween-20 (TBST-MLK) for 1 hour at RT with constant agitation. 3. Incubate the nitrocellulose with 1ug/ml of AIF, A1059-77 diluted in freshly prepared TBS-MLK for three hours with agitation at RT 4. Wash the nitrocellulose twice with water. 5. Incubate the nitrocellulose in the secondary reagent of choice (13000 dilution was used) in TBS-MLK overnight at 4ºC with agitation.
6. Wash the nitrocellulose with water three times.
7. Wash the nitrocellulose in TBS-0.1% Tween 20 for 30 minutes with agitation at RT.
8. Rinse the nitrocellulose in water for 30 minutes with agitation at RT.
9. Use detection method of choice (enhanced chemiluminescence was used).
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
Form200ug of protein A purified rabbit IgG in 200ul of 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 593606 of human Apoptosis Inducing Factor, AIF (C-KDGEQHEDLNEV-AK), containing a N-terminal cysteine. The immunizing sequence is identical in mouse and rat.
SpecificityRecognizes AIF, Mr 57kDa. Another non-specific protein was also detected at Mr 120kDa. Species Crossreactivity: Mouse. Predicted for human and rat based on sequence homology
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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