Anti -Apoptosis Inducing Factor (CT) (AIF, MGC111425, Programmed cell death protein 8,
mitochondrial precursor, PDCD8)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed. | | | Catalog # | A1059-78 | | Applications | Suitable for use in ELISA, Western Blotting. Other applications not tested. | | Recommended Dilution | Western Blotting: 0.25-1ug/ml. | | Optimal dilutions to be determined by the researcher. | | Positive Control | K562 cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid, 100ug in 200ul of PBS, 0.02% sodium azide. | | Purity | Supplied as immunoaffinity chromatography purified IgG | | Immunogen | Raised against a peptide (KDGEQHEDLNEVAK) corresponding to amino acids 593 to 606 of human AIF. This sequence is identical to those of mouse and rat AIF. | | Specificity | Recognizes human AIF (Apoptosis Inducing Factor). Species crossreactivity: Mouse and rat. | | | Important Note | This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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