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You are here:Home » Antibodies » Abs to Apoptosis » Anti -Apoptosis Inducing Factor ((AIF, MGC111425, Programmed cell death protein 8, mitochondrial precursor, PDCD8)

Anti -Apoptosis Inducing Factor ((AIF, MGC111425, Programmed cell death protein 8,
mitochondrial precursor, PDCD8)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Mitochondria play a pivotal role in the regulation of programmed cell death or apoptosis. Apoptosis is driven by two classes of specialized proteases known as caspases (Cysteine aspartase). The initiator caspases can be activated by self-cleavage. The effector caspases are then activated in an amplification cascade. Several key participants are released from the mitochondria that regulate apoptosis. The first such factor (Cytochrome-C) to be described binds to a cytoplasmic scaffolding protein called Apaf-1. Binding of the mitochondria factor allows Apaf-1 to form a ternary complex with, and activate, the initiator pro-caspase-9. Active caspase-9 then turns on downstream effector caspases, initiating apoptosis. Another mitochondrial-derived factor, termed Apoptosis inducing factor (AIF), has been identified. AIF is sufficient to induce apoptosis of isolated nuclei. AIF (mouse 612 aa, rat 613 aa, and human 612 aa, chromosome Xq25-q26) is a flavoprotein of ~57kD. It normally resides in the mitochondrial but translocates to the nucleus. AIF induces mitochondrial to release Cytochrome-c and caspase-9. It has been found in liver and many other tissues.
Catalog #A1059-80A
Mouse and human AIF are 92% homologous. The C-terminus of AIF shares significant homology with several bacterial ferredoxins, and the N-terminus has two mitochondrial localization signal. The N-terminal 1-101aa are cleaved to produce mature AIF.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum, ECL.
ELISA: 1:10,000-50,000
Optimal dilutions to be determined by the researcher.
Control Peptide A0906-92
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
PuritySerum
Immunogen20-aa peptide from mouse AIF
SpecificityMouse control peptide is 95% conserved in rat and 61% in human AIF. No significant sequence homology is detected with other ferredoxins. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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