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You are here:Home » Antibodies » Abs to Apoptosis » Anti -AIF1 (Allograft Inflammatory Factor 1, AIF-1, Ionized Calcium-binding Adapter Molecule 1, Protein G1, G1, IBA1)

Anti -AIF1 (Allograft Inflammatory Factor 1, AIF-1, Ionized Calcium-binding Adapter
Molecule 1, Protein G1, G1, IBA1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
AIF-1 is a 17kD IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis. Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region.
Catalog #A1059-86G
ApplicationsSuitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsPeptide ELISA: 1:32,000
Western Blot: 0.5-1.5ug/ml, using human brain (Frontal cortex) and human peripheral blood mononucleocyte lysates (~17kD).
Immunohistochemistry (Paraffin): 2-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.5% BSA, pH 7.3, 0.02% sodium azide
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide corresponding to C-NKQFLDDPKYSSDED at the internal region of human AIF1 (NP_001614.3). Species Sequence Homology: canine and rat
SpecificityRecognizes human AIF1. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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