Anti -AIF1 (Allograft Inflammatory Factor 1, AIF-1, Ionized Calcium-binding Adapter
Molecule 1, Protein G1, G1, IBA1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH |
|
| AIF-1 is a 17kD IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis. Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region. | | | Catalog # | A1059-86G1 | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:16,000 | | Western Blot: 1-3ug/ml, observed in human lymph node and spleen lysates on ~16kD bands | | Immunohistochemistry (Paraffin): 2-3ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Polyclonal | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to C-YEEKAREKEKP, from human AIF1, at C-terminal (NP_116573.1, NP_001614.3). | | Specificity | Recognizes human AIF1. Species sequence homology: Canine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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