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Anti -AIRE (Autoimmune Regulator, Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein, APECED Protein, APECED)
Anti -AIRE (Autoimmune Regulator, Autoimmune Polyendocrinopathy Candidiasis Ectodermal
Dystrophy Protein, APECED Protein, APECED)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
B IH |
|
| AIRE (Autoimmune Regulator; also APECED) is a 58-60kD member of the PHD Zn-finger family of transcription factors. It appears to have restricted expression, being identified in thymic medullary epithelium, dendritic cells and neutrophils. AIRE regulates the expression of self-antigens in thymus. In particular, genes coding for these antigens are generally inactive, and show minimal H3K4me3 and AcH3 reactivity in their promoters. Human AIRE is 545aa in length. It contains multiple domains, including an HSR domain that mediates homodimerization (aa1-105), an NLS (aa131-133), a SAND domain (aa181-280), and two Zn-finger PHD domains (aa296-343 and 434-475) that bind to nonmethylated histone H3. There are isoforms that show a Trp substitution for aa437-467, a 95aa substitution for aa1-292, and a 75aa substitution for aa377-545. Over aa476-545, human AIRE shares 71% aa identity with mouse AIRE. | | | Catalog # | A1059-88M | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-1ug/ml | | Immunohistochemistry (paraffin): 2.5ug/ml | | Optimal dilutions to be determined by the researcher. | | Positive Control | HEK293 cell lysates | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to C-KDVDLSQPRKGRKP, from the internal region of the protein sequence according to NP_000374.1; NP_000649.1. | | Specificity | Recognizes human AIRE. Species sequence homology: canine, rat.. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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