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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -AIRE (Autoimmune Regulator, Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein, APECED Protein, APECED)

Anti -AIRE (Autoimmune Regulator, Autoimmune Polyendocrinopathy Candidiasis Ectodermal
Dystrophy Protein, APECED Protein, APECED)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IH
AIRE (Autoimmune Regulator; also APECED) is a 58-60kD member of the PHD Zn-finger family of transcription factors. It appears to have restricted expression, being identified in thymic medullary epithelium, dendritic cells and neutrophils. AIRE regulates the expression of self-antigens in thymus. In particular, genes coding for these antigens are generally inactive, and show minimal H3K4me3 and AcH3 reactivity in their promoters. Human AIRE is 545aa in length. It contains multiple domains, including an HSR domain that mediates homodimerization (aa1-105), an NLS (aa131-133), a SAND domain (aa181-280), and two Zn-finger PHD domains (aa296-343 and 434-475) that bind to nonmethylated histone H3. There are isoforms that show a Trp substitution for aa437-467, a 95aa substitution for aa1-292, and a 75aa substitution for aa377-545. Over aa476-545, human AIRE shares 71% aa identity with mouse AIRE.
Catalog #A1059-88P
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.01-0.03ug/ml
Immunohistochemistry (paraffin): 2.5ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHEK293 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-QSMARPAAPFPS, from the C-terminus of the protein sequence according to NP_000374.1; NP_000649.1.
SpecificityRecognizes human AIRE.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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