Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Transcription Factors » Anti -AIRE (Autoimmune Regulator, Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein, APECED Protein)

Anti -AIRE (Autoimmune Regulator, Autoimmune Polyendocrinopathy Candidiasis Ectodermal
Dystrophy Protein, APECED Protein)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
The AIRE gene function is not well defined, however the protein does contain zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described [1]. The longer AIRE-1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE-2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE-3 includes the same exon 1-8-9 sequences as found in AIRE-2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants are differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).
Catalog #A1059-88U
ApplicationsSuitable for use in ELISA, Western Blot and mmunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:32000
Western Blot: 0.01-0.03ug/ml, in transfected HEK293 lysates on ~60kD bands
Immunohistochemistry (Paraffin): 2.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-QSMARPAAPFPS, from human AIRE, at C-terminal (NP_000374.1, NP_000649.1).
SpecificityRecognizes human AIRE.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links