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Anti -AIRE (Autoimmune Regulator, Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein, APECED Protein)
Anti -AIRE (Autoimmune Regulator, Autoimmune Polyendocrinopathy Candidiasis Ectodermal
Dystrophy Protein, APECED Protein)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B |
|
| The AIRE gene function is not well defined, however the protein does contain zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described [1]. The longer AIRE-1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE-2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE-3 includes the same exon 1-8-9 sequences as found in AIRE-2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants are differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). | | | Catalog # | A1059-88W | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:64,000 | | Western Blot: 0.3-1ug/ml, observed in human spleen lysates on ~55kD bands | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to C-KAKPPKKPESSAEQ, from AIRE, at the internal region of the protein (NP_000374.1, NP_000649.1). | | Specificity | Recognizes human AIRE. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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