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You are here:Home » Antibodies » Abs to Catenin » Anti -Ajuba

Anti -Ajuba

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Ajuba (an Urdu word meaning "curiosity") is a 55-60kD member of the Zyxin/Ajuba family of proteins. Ajuba is found in keratinocytes, astrocytes and neurons, and exhibits multiple functions. It shuttles between the nucleus and cytosol, and participates in alpha- catenin:F-actin binding, beta-catenin binding and phosphorylation, p62 aPKC scaffold protein binding and NF- B activation, and Aurora-A binding and phosphorylation. Human Ajuba is 538aa in length. It contains a Pro-rich preLIM region that binds actin and Grb2 (aa1-335), an NES (aa280 -287), and three LIM domains that bind multiple proteins (aa336-397, 401-461 and 462-530). Ajuba is a LIM domain protein suggested to bind and regulate the activity of Aurora A. Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.
Catalog #A1099-01C
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:20,000-1:80,000
Western Blot: 1:500-1:2,500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1.5mg/ml
FormSupplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% (w/v) sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide, CQESRHSYPPALGSPG, corresponding to amino acid 224-239 of human Ajuba.
SpecificityRecognizes human Ajuba at ~57kD. Species sequence homology: human, rat, dog, mouse and chimpanzee 100%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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