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You are here:Home » Antibodies » Antibodies-Catenin » Anti -Ajuba (JUB, MGC15563, ajuba homolog isoform 1)

Anti -Ajuba (JUB, MGC15563, ajuba homolog isoform 1)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP FC
Ajuba belongs to the Zyxin/Ajuba family, members of which include LIMD1, LPP, Trip6 and Zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin/Ajuba family members associate with the actin cytoskeleton, are components of both cell-cell junction adhesive complex and integrin-mediated adhesive complex, and shuttle in and out of the nucleus (1). Ajuba is involved in Rac activation during cell migration and activates Aurora A in order to commit to mitosis (2-3). Ajuba and its family members also have an amino-terminal proline-rich pre-LIM region, and Ajuba augments ERK activation in a Ras-dependent manner (4).
Catalog #A1099-01D
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:100
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenSynthetic peptide corresponding to human Ajuba.
SpecificityRecognizes endogenous levels of total Ajuba protein. Species crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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