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You are here:Home » Antibodies » Antibodies-Protein Kinases » Anti -AKAP, Muscle (mAKAP, A-Kinase Anchor Protein)

Anti -AKAP, Muscle (mAKAP, A-Kinase Anchor Protein)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Catalog #A1102-80
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-4ug/ml of this lot detected mAKAP in RIPA cell lysates from L6 cells.Rat L6 cell lysate was resoved by elecrophoresis, transferred to nitrocellulose and probed with A1102-80 (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitated mAKAP from an L6 RIPA cell lysate.
Immunohistochemistry: Has been shown to detect mAKAP in differentiated rat neonatal ventriculocytes and frozen rat heart tissue.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, 30% glycerol..
PurityPurified by Protein A affinity chromatography.
ImmunogenHis-tagged, recombinant protein corresponding to residues 1666-2319 of human mAKAP (muscle AKAP).
SpecificityRecognizes human mAKAP, Mr ~255kD, in skeletal muscle, ~300kD in brain and heart. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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