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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AKAP, Muscle (mAKAP, A-Kinase Anchor Protein)

Anti -AKAP, Muscle (mAKAP, A-Kinase Anchor Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Catalog #A1102-80
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-4ug/ml of this lot detected mAKAP in RIPA cell lysates from L6 cells.Rat L6 cell lysate was resoved by elecrophoresis, transferred to nitrocellulose and probed with A1102-80 (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitated mAKAP from an L6 RIPA cell lysate.
Immunohistochemistry: Has been shown to detect mAKAP in differentiated rat neonatal ventriculocytes and frozen rat heart tissue.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, 30% glycerol..
PurityPurified by Protein A affinity chromatography.
ImmunogenHis-tagged, recombinant protein corresponding to residues 1666-2319 of human mAKAP (muscle AKAP).
SpecificityRecognizes human mAKAP, Mr ~255kD, in skeletal muscle, ~300kD in brain and heart. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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