| Catalog # | A1102-80 |
| Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-4ug/ml of this lot detected mAKAP in RIPA cell lysates from L6 cells.Rat L6 cell lysate was resoved by elecrophoresis, transferred to nitrocellulose and probed with A1102-80 (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Immunoprecipitation: 4ug immunoprecipitated mAKAP from an L6 RIPA cell lysate. |
| Immunohistochemistry: Has been shown to detect mAKAP in differentiated rat neonatal ventriculocytes and frozen rat heart tissue. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, 30% glycerol.. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | His-tagged, recombinant protein corresponding to residues 1666-2319 of human mAKAP (muscle AKAP). |
| Specificity | Recognizes human mAKAP, Mr ~255kD, in skeletal muscle, ~300kD in brain and heart. Species Crossreactivity: rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
