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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AKAP6 (A Kinase (PRKA) Anchor Protein 6, ADAP6, PRKA6, mAKAP, ADAP100, AKAP100, KIAA0311, A-Kinase Anchor Protein 100)

Anti -AKAP6 (A Kinase (PRKA) Anchor Protein 6, ADAP6, PRKA6, mAKAP, ADAP100, AKAP100,
KIAA0311, A-Kinase Anchor Protein 100)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is highly expressed in various brain regions and cardiac and skeletal muscle. It is specifically localized to the sarcoplasmic reticulum and nuclear membrane, and is involved in anchoring PKA to the nuclear membrane or sarcoplasmic reticulum.
Catalog #A1102-81E
ApplicationsSuitable for use in ELISA. Other Applications not tested.
Recommended DilutionsELISA: 1:16,000
Optimal dilutions to be determined by the researcher
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See A1102-81E-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide, LTMSVTLSPLRSQ-C, from the N-Terminus of human AkAP6.
SpecificityRecognizes human AkAP6. Species sequence homology: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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