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You are here:Home » Molecular Biology » MB-Peptides » AKAP6 (A Kinase (PRKA) Anchor Protein 6, ADAP6, PRKA6, mAKAP, ADAP100, AKAP100, KIAA0311, A-Kinase Anchor Protein 100) (Control Peptide)

AKAP6 (A Kinase (PRKA) Anchor Protein 6, ADAP6, PRKA6, mAKAP, ADAP100, AKAP100, KIAA0311,
A-Kinase Anchor Protein 100) (Control Peptide)

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Specifications

LTMSVTLSPLRSQ-C, from the N-Terminus of human AkAP6.
Control peptide corresponding to polyclonal antibody, Catalog#A1102-81E, goat x human.
Catalog #A1102-81E-P
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is highly expressed in various brain regions and cardiac and skeletal muscle. It is specifically localized to the sarcoplasmic reticulum and nuclear membrane, and is involved in anchoring PKA to the nuclear membrane or sarcoplasmic reticulum.
ApplicationsSuitable for use in ELISA. Other Applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking (see corresponding antibody A1102-81E)Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
PurityHighly Purified
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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