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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AKAP 95 (A Kinase Protein 95)

Anti -AKAP 95 (A Kinase Protein 95)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC
A-kinase proteins (AKAPs) function to localize the cAMP-dependent protein kinase (PKA) to specific subcellular sites. Anchoring of PKA is an efficient means to adapt the kinase for specific cAMP-responsive events as it permits rapid and preferential phosphorylation of target substrates upon elevation of cAMP. So far, 35 unique AKAPs have been identified which function to tether the kinase to a variety of cellular structures and organelles. AKAP95 is a 95kD protein which is a component of the nuclear matrix in all cell-lines.
Catalog #A1105
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml. Detects AKAP95 in human A431 lysates and HeLa nucler extract.
Immunocytochemistry: 10ug/ml. Detect AKAP95 in human A431 cells.
Optimal dilution determined by the researcher.
Included Positive Antigen ControlH1838: HeLa nuclear extract cell lysate.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
HI1904-40D: IgG, H&L (FITC) Pab Gt xRb
I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant mouse AKAP expressed in E. coli
SpecificityRecognizes AKAP 95. Species Crossreactivity: human, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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