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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AKAP 150 (A Kinase Protein 150)

Anti -AKAP 150 (A Kinase Protein 150)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.
Catalog #A1105-25
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:5000 detects AKAP 150 in a rat brain cytosolic preparation (5ug). Rat brain cytosolic preparation (5ug/lane) was resolved by electrophoresis, transferred to nitrocellulose and probed with A1105-25. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot Determined
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
PuritySerum
ImmunogenSynthetic peptide corresponding to aa428-449, C-TTVGQAEEATVGQAEEATVGQA of rat AKAP 150 (KLH).
SpecificityRecognizes rat AKAP 150, Mr 150kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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