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A1105-75A Rabbit Anti-AKAP-associated Sperm Protein (ASP, ROPN1L, Ropporin-1 Like, AKAP-associated Sperm Protein)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC
Crossreactivity
Hu
Accession #
NP_114122
Shipping Temp
Blue Ice
Storage Temp
-20°C

AKAPs act as scaffolding proteins co-ordinating phosphotase:kinase signaling at the cytoskeleton. They interact with PKAs and anchor them to specific sub-cellular compartments and participate in multiple cellular processes including growth, metabolism and differentiation. ASP, a 26KD protein, is a novel AKAP-interacting protein. It interacts with the amphipathic helix of AKAP3 and facilitates AKAP3-PKA interactions. It consists of a conserved N-terminal AKAP-binding domain and a central cysteine-rich Fz domain. It is specifically expressed in the sperms and might play a significant role in sperm motily and also facilitate cytoskeletal reorganization during spermiogenesis.

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa58-73(KDRMEMPTATQKTDTG) and 176-191(ARLDSDVSPLETESYL) of human ASP.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human AKAP-associated sperm protein (ASP). Species sequence homology: mouse, bovine, chimpanzee, canine.
References
1. Carr,D.W., Fujita,A., Stentz,C.L., Liberty,G.A., Olson,G.E. and Narumiya,S. Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA. J. Biol. Chem. 276 (20), 17332-17338 (2001). 2. Anway,M.D., Ravindranath,N., Dym,M. and Griswold,M.D. Identification of a murine testis complementary DNA encoding a homolog to human A-kinase anchoring protein-associated sperm protein. Biol. Reprod. 66 (6), 1755-1761 (2002).
USBio References
No references available
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