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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AKT, phosphorylated (Thr34) (Rac PKa, PKBa)

Anti -AKT, phosphorylated (Thr34) (Rac PKa, PKBa)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
AKT, also known as Protein Kinase B (PKB) and Rac (Related to the A and C kinases), is a 60kDa serine/threonine kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. AKT phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. AKT is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate which may induce the membrane translocation of AKT coincident with its phosphorylation and activation. Upon activation, AKT associates with members of the PKC family of kinases, such as PKCä and PKCæ. Ceramide-activated PKCæ leads to phosphorylation of the AKT-Pleckstrin Homology (PH) domain on threonine 34. This phosphorylation inhibits PIP3 binding to AKT, preventing activation of the kinase by insulin. Since AKT confers a prosurvival signal and regulates pathways in response to insulin, suppressing its activation may be one mechanism by which ceramide promotes cell death and induces insulin resistance.
Catalog #A1124-01M
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlCalyculin A (10nM, 30 minutes) treated A431 cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% gylcerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenPhospho-AKT (Thr34) synthetic peptide corresponding to aa residues around threonine 34 of human AKT (KLH).
SpecificityRecognizes human AKT, phosphorylated at Thr34. Species Crossreactivity: rat, rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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