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You are here:Home » Antibodies » Antibodies-Protein Kinases » Anti -AKT (Rac PKa, PKBa)

Anti -AKT (Rac PKa, PKBa)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP
The protein kinase B (PKB, also called AKT) family of protein kinases plays a key role in insulin signaling, cellular survival, and transformation. AKT1 is activated by phosphorylation on residues threonine 308 and Serine 473. Thr308, in the catalytic domain, is phosphorylated by the protein kinase PDK1, and Ser473, in the COOH-terminal regulatory domain, is phosphorylated by putative serine 473 kinase. To date, a couple of proteins have been shown to physically interact with PKB.
Catalog #A1124-01P
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.1-1ug/ml
Western Blot: 0.5-2ug/ml
Immunoprecipitation: 3-5ug/extract
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminus of AKT1, surrounding Ser473.
SpecificityRecognizes human AKT1. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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