| Akt is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser473 and Thr308. Akt is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). Akt is a key regulator of many signal transduction pathways. Akt exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of Akt is noted in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: inhibition of apoptosis and promotion of proliferation. |
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| Applications: |
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| Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
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| Catalog # | A1124-02B |
| Recommended Dilutions | ELISA: 1:15,000-1:60,000 |
| Western Blot: 1:200-1:1000, Detects a band at ~55.7kD |
| Immunohistochemistry (Formalin-fixed, Paraffin-embedded sections): 1:100-1:500. No pretreatment required. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A synthetic peptide corresponding to the C-terminus of aa460-480 of human, mouse, rat and chicken Akt proteins (KLH). |
| Specificity | Recognizes phosphorylated human Akt. Minimal reactivity occurs against non-phosphorylated Akt. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum. Species Crossreactivity: mouse and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
