Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » MB-Protein Kinases » Akt, Substrate Peptide (Rac PKa, PKBa)

Akt, Substrate Peptide (Rac PKa, PKBa)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

AKT is a serine/threonine kinase that enhances cell proliferation and inhibits apoptosis when
activated. Akt associates with PI(3,4,5)P3 at the cell membrane through its pleckstrin homology domain and is activated through phosphorylation. 3-Phosphoinositide-dependent kinase-1 (PDK1) has been shown to first phosphorylate Akt at Thr308, then a second kinase phosphorylates Akt at position Ser473. In turn, Akt phosphorylates a host of proteins including the proapoptotic proteins BAD and pro-caspase-9, GSK3, p21WAF1, MDM2, and the forkhead (FKHR) family of transcription factors.
Catalog #A1124-07D
Peptide Sequence (linear)R-P-R-A-A-T-F (Arg-Pro-Arg-Ala-Ala-Thr-Phe)
MW817
ApplicationsSuitable for use as a substrate for Protein Kinase B alpha (PKB/AKT; Km = 5uM) Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityStorage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Purity80%
Concentration~1mg/ml
FormSupplied as a lyophilized powder.
SpecificityA specific substrate for Protein Kinase B alpha (PKB/ AKT; Km = 5uM). This peptide is not phosphorylated by p70 S6 kinase or MAP kinase activated protein (MAPKAP) kinase-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links