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Specifications

AKT is a serine/threonine kinase that enhances cell proliferation and inhibits apoptosis when
activated. Akt associates with PI(3,4,5)P3 at the cell membrane through its pleckstrin homology domain and is activated through phosphorylation. 3-Phosphoinositide-dependent kinase-1 (PDK1) has been shown to first phosphorylate Akt at Thr308, then a second kinase phosphorylates Akt at position Ser473. In turn, Akt phosphorylates a host of proteins including the proapoptotic proteins BAD and pro-caspase-9, GSK3, p21WAF1, MDM2, and the forkhead (FKHR) family of transcription factors.
Catalog #	A1124-07D
Peptide Sequence (linear)	R-P-R-A-A-T-F (Arg-Pro-Arg-Ala-Ala-Thr-Phe)
MW	817
Applications	Suitable for use as a substrate for Protein Kinase B alpha (PKB/AKT; Km = 5uM) Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	Storage and Stability:
	Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Purity	80%
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder.
Specificity	A specific substrate for Protein Kinase B alpha (PKB/ AKT; Km = 5uM). This peptide is not phosphorylated by p70 S6 kinase or MAP kinase activated protein (MAPKAP) kinase-1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.