Anti -Akt, phosphorylated (Ser473) (Protein kinase B, RAC-alpha serine/threonine-protein
kinase, murine thymoma viral (v-akt) oncogene homolog-1, rac protein kinase alpha, v-akt
murine thymoma viral oncogene homolog 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH FC IF |
|
| Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including glucose metabolism, cell survival/apoptosis, cell cycle control, angiogenesis, differentiation and cell growth and proliferation. In mammals three isoforms of Akt (Akt1/PKB alpha, Akt2/PKB beta, and Akt3/PKB gamma) exist. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin- responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module to direct Akt to PIP3 at the cell membrane that is necessary for its activation, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of Akt is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively. | | | Catalog # | A1124-90A | | Applications | Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 2ug/ml | | Flow Cytometry: 1:800 | | Western Blot: 0.5-1ug/ml detected phosphorylated AKT in lysates from mouse NIH-3T3 fibroblasts treated with 100ng/ml PDGF for 20 minutes. | | Immunohistochemistry: 1:200; Pretreatment with citrate, pH 6 antigen retrieval. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 9i145 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Peptide encompassing and including phosphorylated Ser473 of human Akt1. | | Specificity | Recognizes human Akt at ~60kD only when phosphorylated on Ser473. Does not crossreact with Akt1/PKBalpha when it is not phosphorylated on Ser473. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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