Akt1, Human, phosphorylated, active, Western Blot Positive Control (v-akt Mouse Thymoma
Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA,
RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) Recombinant
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| Putative human homologs of the protooncogene v-akt of the acutely transforming retrovirus AKT8 have been cloned. These protein-serine/threonine kinase proteins have a catalytic domain closely related to both PKA and PKC and have been designated rac (related to A and C kinases), pkb (Protein kinase B) or Akt. RAC protein kinase family members feature pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the C-terminus. The N-terminal domain (referred to as AH-Akt Homology domain) spans from 1-148 amino acids and contains the PH domain, a region found in diverse group of signaling proteins. The PH domain (aa1-106) has been implicated in interactions with other proteins such as G-protein bg subunits, as well as phosphoinositides. The kinase domain is located between residues 148-411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells. | | | Catalog # | A1125-01 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: Load ~10 ul/lane. | | Optimal dilutions to be determined by researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6-12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | Human, recombinant protein expressed in sf21 cell. | | Purity | Highly purified ( 90%). | | Concentration | Not determined | | Form | Supplied in SDS-PAGE sample buffer (reduced). This preparation of AKT1 is phosphorylated but not active in sample buffer. | | Specificity | This preparation was active (Specific Activity: 300-1800u/mg). AKT1 was activated with MAPKAP kinase 2 and PDK1 and repurified. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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