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You are here:Home » Molecular Biology » MB-Protein Kinases » Akt1, Human, unactive, Western Blot Positive Control (v-akt Mouse Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) Recombinant

Akt1, Human, unactive, Western Blot Positive Control (v-akt Mouse Thymoma Viral Oncogene
Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha
serine/threonine-protein kinase, RAC-PK-alpha) Recombinant

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Specifications

Putative human homologs of the protooncogene v-akt of the acutely transforming retrovirus AKT8 have been cloned. These protein-serine/threonine kinase proteins have a catalytic domain closely related to both PKA and PKC and have been designated rac (related to A and C kinases), pkb (Protein kinase B) or Akt. RAC protein kinase family members feature pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the C-terminus. The N-terminal domain (referred to as AH-Akt Homology domain) spans from 1-148 amino acids and contains the PH domain, a region found in diverse group of signaling proteins. The PH domain (aa1-106) has been implicated in interactions with other proteins such as G-protein bg subunits, as well as phosphoinositides. The kinase domain is located between residues 148-411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells.
Catalog #A1125-01A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: Load ~10 ul/lane.
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6-12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceHuman, recombinant protein expressed in sf21 cell.
PurityHighly purified ( 90%).
ConcentrationNot determined
FormSupplied in SDS-PAGE sample buffer (reduced).
SpecificityThis preparation of AKT1 is inactive or minimally active. It has not been phosphorylated or dephosphorylated. It may have some basal level of phosphorylation.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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