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You are here:Home » Molecular Biology » MB-Protein Kinases » Akt1, phosphorylated (Ser472), Rat, Control Peptide (Rac PKa, PKBa)

Akt1, phosphorylated (Ser472), Rat, Control Peptide (Rac PKa, PKBa)

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Specifications

Rat AKT1 peptide (~C-terminus, 15 aa) containing a phosphorylated Ser472coupled to KLH
Catalog #A1125-01B
Putative human homolog of the proto-oncogene v-akt of the acutely transforming retrovirus AKT8 have been cloned. These protein-serine/threonine kinase proteins have a catalytic domain closely related to both PKA and PKC and have been designated rac (related to A and C kinases), pkb (Protein kinase B) or Akt.
RAC protein kinase family members feature pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus. The Amino terminal domain (referred to as AH-Akt Homology domain) spans from 1-148 amino acids and contains the PH domain, a region found in diverse group of signaling proteins. The PH domain (amino acids 1-106) has been implicated in interactions with other proteins such as G-protein bg subunits, as well as phosphoinositides. The kinase domain is located between residues 148 to 411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells.
AKT1 (RAC-PK-a or PKB-a) is the human homolog of v-akt and is identical to RAC gene. The protein has been observed to show different migratory patterns on a western blot according to the state of phosphorylation of the protein. Phosphatase treatment has been shown to result in inactivation of the protein.
ApplicationSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA (1:10K-1:100K; using 50-100 ng of control peptide/well).
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityPurified by affinity chromatography.
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
SpecificityRat phospho-peptide is 93% homologous in Mouse/ human/ chicken and bovine. The control immunogenic phopsho-peptide as well as the non-phospho peptide are available to confirm specificity of antibodies. The control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of affinity purified IgG or 1 ul antiserum) to confirm antibody specificity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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