Atk1, inactive, Recombinant, Human, N-terminal His tag, Serine/Threonine Kinase
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| Human Akt1 (GenBank Accession No. NM_001014432), (a.a. 1-480) with N-terminal HIS6 tag, MW 60.2kD, expressed in an Baculovirus infected Sf9 cell expression system. | | | Catalog # | A1125-01D | | Application | useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. | | Source | Baculovirus infected Sf9 cells | | Form | 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT. | | Purity | 80% by SDS-PAGE. | | Specific Activity | 100U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of Crosstide substrate per minute at pH 7.4 and 30°C. | | Assay buffer | 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.1mM ATP. | | Storage and Stability | Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. | | Source | Recombinant, human from Baculovirus infected Sf9 cells | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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