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You are here:Home » Molecular Biology » MB-Protein Kinases » Atk1, inactive, Recombinant, Human, N-terminal His-Tag, Serine/Threonine Kinase

Atk1, inactive, Recombinant, Human, N-terminal His-Tag, Serine/Threonine Kinase

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Specifications

Human Akt1 (GenBank Accession No. NM_001014432), (a.a. 1-480) with N-terminal HIS6 tag, MW 60.2kD, expressed in an Baculovirus infected Sf9 cell expression system.
Catalog #A1125-01D
Application useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Source Baculovirus infected Sf9 cells
Form 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT.
Purity 80% by SDS-PAGE.
Specific Activity 100U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of Crosstide substrate per minute at pH 7.4 and 30°C.
Assay buffer 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.1mM ATP.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
SourceRecombinant, human from Baculovirus infected Sf9 cells
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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