Akt1, active, Recombinant, Human (Rac PKa, PKBa)
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| Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal GST tag, co-expressed with PDK1 in an Baculovirus infected Sf9 cell expression system. | | | Catalog # | A1125-01L | | Specific Activity | ~12pmol/min/ug | | Assay Conditons | Enzyme reaction is conducted in a buffer | | containing 50mM HEPES, pH 7.5, 10mM | | magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, and 2uM substrate (SER/THR 6) at RT for 1 hour. | | Applications | Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. | | Recommended Dilutions | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. | | Molecular Weight | 60.2kD | | Source | Sf9 insect cells | | Purity | 70% (SDS-PAGE) | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20, 3mM DTT, 50% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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