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You are here:Home » Molecular Biology » MB-Protein Kinases » Akt1, active, Recombinant, Human, GST-Tag (Rac PKa, PKBa)

Akt1, active, Recombinant, Human, GST-Tag (Rac PKa, PKBa)

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Specifications

Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal GST tag, co-expressed with PDK1 in an Baculovirus infected Sf9 cell expression system.
Catalog #A1125-01L
Specific Activity~12pmol/min/ug
Assay ConditonsEnzyme reaction is conducted in a buffer
containing 50mM HEPES, pH 7.5, 10mM
magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, and 2uM substrate (SER/THR 6) at RT for 1 hour.
ApplicationsSuitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight60.2kD
SourceSf9 insect cells
Purity70% (SDS-PAGE)
Concentration~0.2mg/ml
FormSupplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20, 3mM DTT, 50% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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